Here you can find the lab protocols for using the Rps10 barcode for amplicon metagenomics (aka metabarcoding) and generating sequences to contribute to the database. Note that the primers used to generate amplicons short enough for high-throughput sequencing are not the same as those used to generate the database. Make sure you are using the correct protocol for you goals.

Metagenomics protocols

These relate to using the Rps10 barcode for metagenomics research.

Rps10 metabarcoding primer ordering and mixing

This protocol is used to prepare PCR primers for the rps10 locus for metabarcoding.

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Rps10 Locus-specific Illumina Amplicon PCR

This protocol is used to PCR amplify the Rps10 locus for use in amplicon metagenomics research.

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Database protocols

These relate to generating sequences to contribute to the Rps10 reference database hosted on this website.

Rps10 database sequence PCR protocol

This protocol is used to generate sequences for the Rps10 reference database.

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© Copyright 2018 USDA Agricultural Research Service and the Grunwald Lab

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